《植物生理学报》 2009, 45(4): 329-332

生长素类物质对植原体感染酸枣组培苗的影响

孙清荣^1,*, 孙洪雁¹, 王妍², 刘嘉芬¹, 周广芳¹, 刘庆忠¹

1 山东省果树研究所, 农业部泰安温带果树资源重点野外科学观测试验站, 山东省果树生物技术育种重点实验室, 山东泰安271000; 2 山东农业大学园艺学院, 山东泰安 271000

通信作者：孙清荣；E-mail: qingrongsun@hotmail.com；Tel: 0538-8282059

摘　要：

观察生长素类物质对感染植原体病酸枣试管苗影响的结果表明: 添加外源 NAA 和 IBA 后的病苗丛枝症状和不加生 长素类物质相似, 均表现伸长生长和梢较上部位有多个分枝梢生长, 而在培养基中添加IAA后可使病苗从其基部产生更多 丛生芽。培养 9 个月后, 用 NAA、IBA 和 IAA 处理的都没有检测出植原体病原脱除的绿苗, 也没有观察到丛生症状减轻的 病苗。

关键词：生长素; 植原体; 酸枣; 组培苗

收稿：2008-11-27   修定：2009-01-21

资助：山东省农科院博士科研启动基金(2007YBS004)、国家林业科技支撑计划(2006BAD01A1701、2006BAD18B02)和山东省农业良种工程项目(2005LZ07-01、2006LZ07-01)。

Effects of Auxin Analogs on Phytoplasma in Infected Sour Jujube Shoots Grown in vitro

SUN Qing-Rong^1,*, SUN Hong-Yan¹, WANG Yan², LIU Jia-Fen¹, ZHOU Guang-Fang¹, LIU Qing-Zhong¹

¹Shandong Fruits Biotechnology Breeding Key Lab, The State Agriculture Ministry Experiment Station of Temperate Fruits Germplasm Key Outdoor Observation in Taian, Shandong Institute of Pomology, Taian, Shandong 271000, China; ²College of Horticulture, Shandong Agricultural University, Taian, Shandong 271018, China

Corresponding author: SUN Qing-Rong; E-mail: qingrongsun@hotmail.com; Tel: 0538-8282059

Abstract:

Sour jujube witches’ broom is resulted from imbalance of endogeneous hormones, which is caused by phytoplasma. In order to investigate the effects of auxin analogs on witches’ broom phytoplasma, the infected shoots of sour jujube (Zizyphus spinosus Hu.) were used as materials. The infected shoots were treated with exogenous auxin analogs NAA, IBA and IAA, respectively. The results showed that NAA and IBA didn’t affect witches’ broom, and IAA promoted shoot clumps production. After the treatments of NAA, IBA and IAA for 9 months, PCR analysis of phytoplasma presence showed the pathogen of witches’ broom phytoplasma was not been eliminated in tested shoots, remission of witches’ broom symptom was not observed in all treatments.

Key words: auxin analogs; phytoplasma; sour jujube; in vitro shoots